Coding
gldA

Part:BBa_K1172201:Design

Designed by: Lukas Rositzka   Group: iGEM13_Bielefeld-Germany   (2013-08-28)

GldA - glycerol dehydrogenase from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplification with Gibson-primer:

  • Amplification of the glycerol dehydrogenase (gldA):
Forward primer gldA (43 bp)

5'-GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC-3'

Reverse primer gldA (45 bp)

5'-CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG-3'
  • Amplification of the pSB1C3-Backbone:
Forward primer pSB1C3 gldA (43 bp)

5'-TCCTGCAAGAGTGGGAATAATACTAGTAGCGGCCGCTGCAGTC-3'

Reverse primer pSB1C3 gldA (43 bp)

5'-GATTGAATAATGCGGTCCATCTAGAAGCGGCCGCGAATTCCAG-3'


Ligation via [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Gibson_assembly Gibson-Assembly].


Source

Escherichicha coli KRX genomic sequence ([http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Whole_Genome_Isolation Whole Genome Isolation])


References